Living cells tracer kit (green fluorescence) Other kits Serial number Possible cause Solutions

Serial number Possible cause Solutions 1 Less washing times

and 1 mL of the cell broth is added

MGMT is expressed in highly variable amounts

and the actual concentration of gentamicin in the sample was multiplied by its corresponding dilution

and 100u L (lnput)

Living cells tracer kit (green fluorescence) Other kits Serial number Possible cause SolutionsProduct Specification Usage Self brought consumables: 24 well plate, adjustable pipette gun and tip Centrifuge Fluorescence microscope or flow cytometry Deionized water, PBS Reagent Preparation: Cell Tracker GreenOperate on ice, protect from light and store in separate packages to avoid repeated freezing and thawing. 1 Assay BufferDilute 5 Assay Buffer to 1 Assay Buffer with deionized water. Heat to 37 C before use. Staining SolutionPrior to the start