Human RIPK3 ELISA Kit Enzymes and Regulators dual-wavelength detection was performed using

dual-wavelength detection was performed using a microplate reader to determine 450nm Absorption maximum wavelength and 570nm Or 630nm At reference wavelength OD Value

Nucleus Accession Q9UJQ4 Clone Number SDT-1484-1 Antibody Type Recombinant mAb Isotype IgG Application WB

Theory Based on the caspase 2 specific hydrolysis of peptide substrate Ac-VDQQD-pNA (Val Asp Val Ala Asp p nitroanilide)

Y84S) PKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNNASRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Expression System HEK293 Molecular Weight 30-33kDa Purity >95% by SDS-PAGE Endotoxin <0

Ferritin heavy chain

Human RIPK3 ELISA Kit Enzymes and Regulators dual-wavelength detection was performed usingProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and