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Setting of positive control:
it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted within 1-2min
seal with cover glass
The number of blue cells and total cells was counted
Mitogen-activated protein kinase kinases 1 and 2 (MEK1/2) are the crucial part of the RAS-RAF-MEK-ERK pathway (or ERK pathway)
Human IgG1 F(c) Mouse mAb (S-448-1) Porcine ELISA Kit Setting of positive control:Product Specification Host Mouse Antigen Human IgG1 F(c) Synonyms Immunoglobulin heavy constant gamma 1, Ig gamma 1 chain C region, Ig gamma 1 chain C region EU, Ig gamma 1 chain C region KOL, Ig gamma 1 chain C region NIE, IGHG1 Immunogen Synthetic Peptide Location Secreted, Cell membrane Accession P01857 Clone Number S 448 1 Antibody Type Mouse mAb Isotype IgG1 Application ELISA, WB, IHC P Reactivity Hu Purification Protein G Concentration 2 mg ml