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whereas the 89-kDa PARP1 fragment is translocated to the cytoplasm [PMID: 11570811
Death Receptor 3 (DR3)
and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Cell Division Cycle Protein 25 (cdc25a)
dilute it appropriately based on the actual concentration (a pilot experiment is recommended to determine the dilution factor)
application dilution species Dot Blot 1:1000 WB 1:1000 Ms
Rat EPO ELISA Kit Drug Target Proteins whereas the 89-kDa PARP1 fragmentProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
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